2017-04-24 15:34:122025-05-11 10:41:55
description
K /H antiporter for K efflux
K+/H+ antiporter for K+ efflux
locus
BSU09850
BSU_09850
proteinLength
408
405
geneLength
1224
1218
product
K /H antiporter for K efflux
K+/H+ antiporter for K+ efflux
ec
outlinks
bsu
BSU09850
BSU_09850
Gene
Coordinates
1,059,203 → 1,060,420
1,059,203 1,060,420
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
cell membrane, integral membrane protein (according to UniProt)
Biological materials
Mutant
MGNA-A682 (yhaU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/682 NBRP B. subtilis, Japan]
GP2078 (''[[gene|khtS]]-[[gene|khtT]]-[[gene|khtU]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
MGNA-A682 (yhaU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/682 NBRP B. subtilis, Japan]
GP2078 ([[gene|khtS]]-[[gene|khtT]]-[[gene|khtU]]::''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE09850 ([[gene|khtU]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
BKK09850 ([[gene|khtU]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
References
Original publications
The protein
Protein family
Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family (with [[protein|CpaA]], according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|4BWZ] (sodium proton antiporter NapA from Thermus thermophilus, 26% identity) [pubmed|23995679]
Biological materials
FLAG-tag construct
GP2432 ''khtU-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab